glyceraldehyde 3 phosphate dehydrogenase gapdh (TaKaRa)
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TaKaRa
glyceraldehyde 3 phosphate dehydrogenase gapdh
Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+gapdh+cdna/qPCR+Human+Reference+cDNA%2C+Oligo(dT)-primed/pm40185258-175-11-14
Average 94 stars, based on 33 article reviews
Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+gapdh+cdna/qPCR+Human+Reference+cDNA%2C+Oligo(dT)-primed/pm40185258-175-11-14
Average 94 stars, based on 33 article reviews
glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars,
2026-09
94/100 stars
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Membrane:Article Title: A paradoxical reduction in susceptibility to colonic injury upon targeted transgenic ablation of goblet cells Article Snippet: .. The membrane was probed with a 32 P-labeled probe — either a 700-bp fragment of DT-A , an 800-bp fragment of 5′-end mITF , or Polymerase Chain Reaction:Article Title: Regulated expression and intracellular localization of cystatin F in human U937 cells. Article Snippet: Cystatin F is a cysteine peptidase inhibitor recently discovered in haematopoietic cells by cDNA cloning.. To further investigate the expression, distribution and properties of the native human inhibitor the promyeloid cell line U937 has been studied.. The cells expressed relatively large quantities of cystatin F, which was found both secreted and intracellularly. Amplification:Article Title: Regulated expression and intracellular localization of cystatin F in human U937 cells. Article Snippet: Cystatin F is a cysteine peptidase inhibitor recently discovered in haematopoietic cells by cDNA cloning.. To further investigate the expression, distribution and properties of the native human inhibitor the promyeloid cell line U937 has been studied.. The cells expressed relatively large quantities of cystatin F, which was found both secreted and intracellularly. Article Title: The effect of C-peptide on cognitive dysfunction and hippocampal apoptosis in type 1 diabetic rats. Article Snippet: .. A primer set for Expressing:Article Title: Accelerated fibrosis and collagen deposition develop in the renal interstitium of angiotensin type 2 receptor null mutant mice during ureteral obstruction. Article Snippet: .. Detection of a1(I) collagen mRNA expression in five-day obstructed kidneys Fifteen micrograms of total RNA extracted from kidney homogenates was used for Northern blot analysis probed with rat a1(I) cDNA [24] and Northern Blot:Article Title: Accelerated fibrosis and collagen deposition develop in the renal interstitium of angiotensin type 2 receptor null mutant mice during ureteral obstruction. Article Snippet: .. Detection of a1(I) collagen mRNA expression in five-day obstructed kidneys Fifteen micrograms of total RNA extracted from kidney homogenates was used for Northern blot analysis probed with rat a1(I) cDNA [24] and Article Title: DNA methyltransferase levels and altered CpG methylation in the total genome and in the GSTP1 gene in human glioma cells transfected with sense and antisense DNA methyltransferase cDNA Article Snippet: This study examines the efficacy of using plasmid expression vectors containing sense and antisense DNA MTase cDNA to both upand downregulate intracellular DNA MTase levels in human glioma cells.. The effects of the changes in MTase levels on global genomic DNA methylation and on the methylation status of CpG dinucleotides in the GSTP1 gene were determined in a glioma cell line that overexpresses the GSTP1 gene.. In cells transfected with sense DNA MTase cDNA, MTase gene transcripts increased to a maximum of 2.5-fold at 24 h, while MTase activity increased to a maximum of 3.6-fold at 48 h. The effects of antisense MTase cDNA transfections were less pronounced, and levels of MTase gene transcripts and enzyme activity in transfectants were decreased to only, approximately, one-half the levels of controls. Control:Article Title: Accelerated fibrosis and collagen deposition develop in the renal interstitium of angiotensin type 2 receptor null mutant mice during ureteral obstruction. Article Snippet: .. Detection of a1(I) collagen mRNA expression in five-day obstructed kidneys Fifteen micrograms of total RNA extracted from kidney homogenates was used for Northern blot analysis probed with rat a1(I) cDNA [24] and Article Title: The effect of C-peptide on cognitive dysfunction and hippocampal apoptosis in type 1 diabetic rats. Article Snippet: .. A primer set for Article Title: DNA methyltransferase levels and altered CpG methylation in the total genome and in the GSTP1 gene in human glioma cells transfected with sense and antisense DNA methyltransferase cDNA Article Snippet: This study examines the efficacy of using plasmid expression vectors containing sense and antisense DNA MTase cDNA to both upand downregulate intracellular DNA MTase levels in human glioma cells.. The effects of the changes in MTase levels on global genomic DNA methylation and on the methylation status of CpG dinucleotides in the GSTP1 gene were determined in a glioma cell line that overexpresses the GSTP1 gene.. In cells transfected with sense DNA MTase cDNA, MTase gene transcripts increased to a maximum of 2.5-fold at 24 h, while MTase activity increased to a maximum of 3.6-fold at 48 h. The effects of antisense MTase cDNA transfections were less pronounced, and levels of MTase gene transcripts and enzyme activity in transfectants were decreased to only, approximately, one-half the levels of controls. |
![Figure 3. [U-2H]-pyruvate can noninvasively monitor TERT expression in vivo. FOXO1 transcription factor activity (A), NAMPT activity (B), and GAPDH activity (C) in glioblastoma, oligodendroglioma, and gliosis biopsies. D, Representative 2H-MR spectra from TERTþ and TERT BT88 cells cultured in medium containing 10 mmol/L [U-2H]-pyruvate for 72 hours. A representative spectrum acquired from neat culture medium containing 10 mmol/L [U-2H]-pyruvate is also shown. Peaks for semiheavy water (HDO; 4.75 ppm), pyruvate (2.4 ppm), and lactate (1.3 ppm) are labeled. E, Effect of TERT silencing on 2H-lactate production from [U-2H]- pyruvate in glioblastoma (GBM1), oligodendroglioma (BT88), melanoma (A375), hepatocellular carcinoma (HepG2), and neuroblastoma (SK-N-SH) cells. F, Representative 2H-MR spectral array acquired from a saline-treated mouse bearing a subcutaneous HepG2dox-TERT tumor following intravenous injection of [U-2H]-pyruvate. The first spectrum is acquired prior to [U-2H]-pyruvate injection. Inset shows an expansion of the spectrum with the highest lactate signal (second spectrum following [U-2H]-pyruvate injection). G, Concentration of 2H-lactate produced from [U-2H]-pyruvate at day 0 and day 7 in saline- and doxycycline (DOX)- treated mice bearing subcutaneous HepG2dox-TERT tumors. H, Tumor volume at day 0 and day 7 in saline- and doxycycline (DOX)-treated mice bearing subcutaneous HepG2dox-TERT tumors. Bars depict mean values and error bars represent SD. , P < 0.01; , P < 0.001; , P < 0.0001.](https://doi-unpaywalled-images-cdn.bioz.com/5542/10__1158_slash_1078___0432__ccr___21___4418/10__1158_slash_1078___0432__ccr___21___4418____page6_image1.jpg)
